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mouse rat fgf21 quantikine elisa kit  (R&D Systems)


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    Structured Review

    R&D Systems mouse rat fgf21 quantikine elisa kit
    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
    Mouse Rat Fgf21 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fgf21/Mouse%2FRat+FGF-21+Quantikine+ELISA+Kit/bio_rxiv__64898__2026__03__31__715667-55-22-29
    Average 96 stars, based on 300 article reviews
    mouse rat fgf21 quantikine elisa kit - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice"

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    Journal: bioRxiv

    doi: 10.64898/2026.03.31.715667

    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
    Figure Legend Snippet: (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Techniques Used: Activity Assay, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control

    Related Articles

    other:

    Article Title: HRD1‐ERAD controls production of the hepatokine FGF21 through CREBH polyubiquitination
    Article Snippet: K6 (22900), K11(22901), K27(22902), K29(22903), K33(17607), K48(17605), and K63(17606) ubiquitin plasmids were purchase from addgene.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice
    Article Snippet: Mouse GDF15 was measured using a DuoSet ELISA (R&D Systems product code DY6385) which had been modified to run as an electrochemiluminescence assay on the MesoScale Discovery assay platform. .. Mouse FGF21 was analysed using a Quantikine ELISA kit (R&D Systems, product code MF2100) following the manufacturer’s instructions. .. ALT, AST, Cholesterol and triglycerides were measured on the Siemens Dimension EXL autoanalyser using Siemens reagents and calibrators.

    Article Title: Glucosamine Enhancement of Learning and Memory Functions by Promoting Fibroblast Growth Factor 21 Production.
    Article Snippet: The membrane was exposed to film, and the protein bands were visualized and quantified using the ImageQuant 5.2 software (Molecular Dynamics, Sunnyvale, CA, USA). .. The concentration of FGF21 in the culture medium was determined using an ELISA kit for mouse FGF21 (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Total cellular RNAs were extracted from the harvested tissues and treated HT22 or STHdhQ7/Q7 cells using Tri-reagent according to the manufacturer’s protocol (Sigma).

    Article Title: GDF15 Provides an Endocrine Signal of Nutritional Stress in Mice and Humans
    Article Snippet: Mouse GDF15 was measured using a Mouse GDF15 DuoSet ELISA (R&D Systems) which had been modified to run as an electrochemiluminescence assay on the Meso Scale Discovery assay platform. .. Mouse FGF21 was analyzed by FGF21 Quantakine ELISA (R&D Systems) following the manufacturer’s instructions. ..

    Article Title: Glucosamine Enhancement of Learning and Memory Functions by Promoting Fibroblast Growth Factor 21 Production
    Article Snippet: The membrane was exposed to film, and the protein bands were visualized and quantified using the ImageQuant 5.2 software (Molecular Dynamics, Sunnyvale, CA, USA). .. The concentration of FGF21 in the culture medium was determined using an ELISA kit for mouse FGF21 (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Total cellular RNAs were extracted from the harvested tissues and treated HT22 or STHdh Q7/Q7 cells using Tri-reagent according to the manufacturer’s protocol (Sigma).

    Concentration Assay:

    Article Title: Glucosamine Enhancement of Learning and Memory Functions by Promoting Fibroblast Growth Factor 21 Production.
    Article Snippet: The membrane was exposed to film, and the protein bands were visualized and quantified using the ImageQuant 5.2 software (Molecular Dynamics, Sunnyvale, CA, USA). .. The concentration of FGF21 in the culture medium was determined using an ELISA kit for mouse FGF21 (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Total cellular RNAs were extracted from the harvested tissues and treated HT22 or STHdhQ7/Q7 cells using Tri-reagent according to the manufacturer’s protocol (Sigma).

    Article Title: Glucosamine Enhancement of Learning and Memory Functions by Promoting Fibroblast Growth Factor 21 Production
    Article Snippet: The membrane was exposed to film, and the protein bands were visualized and quantified using the ImageQuant 5.2 software (Molecular Dynamics, Sunnyvale, CA, USA). .. The concentration of FGF21 in the culture medium was determined using an ELISA kit for mouse FGF21 (R&D systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. Total cellular RNAs were extracted from the harvested tissues and treated HT22 or STHdh Q7/Q7 cells using Tri-reagent according to the manufacturer’s protocol (Sigma).



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    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating <t>FGF21</t> expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).
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    Image Search Results


    (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Journal: bioRxiv

    Article Title: Female resistance to the metabolic benefits of protein restriction is reversed by ovariectomy in mice

    doi: 10.64898/2026.03.31.715667

    Figure Lengend Snippet: (A) ANCOVA of energy expenditure (EE) with body weight as a covariate during the 12-hour dark cycle. (B) Average EE during the dark cycle normalized to body weight. (C) Respiratory Exchange Ratio (RER) over a 24-hour period. (D) Average RER during the dark cycle. (E) Spontaneous activity over a 24-hour period. (F) Average spontaneous activity during the dark cycle, calculated as laser beam breaks. (G) Circulating FGF21 expression quantified from refed plasma using ELISA. (H) The mRNA expression of Fgf21 in the liver of mice. (A-F) n=11-12 mice per group, (G-H) n=3-5 mice per group. (A) Data for each individual mouse is plotted, simple linear regression (ANCOVA) was calculated to determine if the slopes or elevations are equal. (B, D, F-H) Statistics for the overall effects of diet, gonadectomy, and the interaction represent the p value from a two-way ANOVA. *p<0.05 from a Šidák’s post-test examining the effect of parameters identified as significant in the two-way ANOVA. Data are represented as mean ±SEM. Abbreviations: CTL (21% control protein diet), LP (7% low protein diet), Cast (castration), Fem (female), Ovx (ovariectomy), BW (body weight), EE (energy expenditure), RER (respiratory exchange ratio), FGF21 (fibroblast growth factor 21).

    Article Snippet: Blood for circulating FGF21, testosterone, and estradiol levels was collected from submandibular bleeding immediately prior to euthanasia; plasma was assayed with a mouse/rat FGF21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA), and testosterone (582701) and estradiol (501890) quantikine ELISA kits from Caymen Chemicals (Ann Arbor, MI, USA).

    Techniques: Activity Assay, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Control